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Addgene inc 105522)
105522), supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/105522)/pGEMHE-mCherry-mTrim21+(Plasmid+%23105522)/pm41862641-327-14-12
Average 93 stars, based on 27 article reviews
105522) - by Bioz Stars, 2026-09
93/100 stars

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Related Articles

other:

Article Title: TBP regulates transposable element expression in early mouse embryos.
Article Snippet: In vitro transcription and antibody purification The mCherry-Trim21 plasmid was obtained from Addgene (# 105522) (Clift et al, 2017). mRNA was transcribed in vitro from the T7 promoter using the mMESSAGE mMACHINE T7 Ultra kit (Ambion #AM1345).

Microinjection:

Article Title: Autophagy regulates the maternal-to-zygotic transition through MAP1LC3B-mediated maternal mRNA decay
Article Snippet: .. To prepare mRNAs for microinjection, pGEMHE-mCherry-mTrim21 (Addgene, 105,522) vectors were linearized and purified with phenol – chloroform, followed by ethanol precipitation. .. Linearized DNAs were transcribed and added to poly (A) tails (200–250 bp) in vitro using the HiScribe T7 ARCA mRNA Kit (with tailing; NEB, E2060S), recovered by lithium chloride precipitation, and resuspended in nuclease-free water.

Article Title: Autophagy regulates the maternal-to-zygotic transition through MAP1LC3B-mediated maternal mRNA decay.
Article Snippet: During the maternal-to-zygotic transition (MZT), the programmed decay of maternal mRNAs is critical for successful embryonic development.. Although autophagy is known to participate in early embryonic development, its specific role in maternal mRNA clearance remains unclear.. MAP1LC3B/LC3B, a key autophagy-related protein, has recently been identified as an RNA-binding protein; however, whether it contributes to maternal mRNA degradation has not been established.

Purification:

Article Title: Autophagy regulates the maternal-to-zygotic transition through MAP1LC3B-mediated maternal mRNA decay
Article Snippet: .. To prepare mRNAs for microinjection, pGEMHE-mCherry-mTrim21 (Addgene, 105,522) vectors were linearized and purified with phenol – chloroform, followed by ethanol precipitation. .. Linearized DNAs were transcribed and added to poly (A) tails (200–250 bp) in vitro using the HiScribe T7 ARCA mRNA Kit (with tailing; NEB, E2060S), recovered by lithium chloride precipitation, and resuspended in nuclease-free water.

Article Title: Autophagy regulates the maternal-to-zygotic transition through MAP1LC3B-mediated maternal mRNA decay.
Article Snippet: During the maternal-to-zygotic transition (MZT), the programmed decay of maternal mRNAs is critical for successful embryonic development.. Although autophagy is known to participate in early embryonic development, its specific role in maternal mRNA clearance remains unclear.. MAP1LC3B/LC3B, a key autophagy-related protein, has recently been identified as an RNA-binding protein; however, whether it contributes to maternal mRNA degradation has not been established.

Ethanol Precipitation:

Article Title: Autophagy regulates the maternal-to-zygotic transition through MAP1LC3B-mediated maternal mRNA decay
Article Snippet: .. To prepare mRNAs for microinjection, pGEMHE-mCherry-mTrim21 (Addgene, 105,522) vectors were linearized and purified with phenol – chloroform, followed by ethanol precipitation. .. Linearized DNAs were transcribed and added to poly (A) tails (200–250 bp) in vitro using the HiScribe T7 ARCA mRNA Kit (with tailing; NEB, E2060S), recovered by lithium chloride precipitation, and resuspended in nuclease-free water.

Article Title: Autophagy regulates the maternal-to-zygotic transition through MAP1LC3B-mediated maternal mRNA decay.
Article Snippet: During the maternal-to-zygotic transition (MZT), the programmed decay of maternal mRNAs is critical for successful embryonic development.. Although autophagy is known to participate in early embryonic development, its specific role in maternal mRNA clearance remains unclear.. MAP1LC3B/LC3B, a key autophagy-related protein, has recently been identified as an RNA-binding protein; however, whether it contributes to maternal mRNA degradation has not been established.

Plasmid Preparation:

Article Title: MRCK activates mouse oocyte myosin II for spindle rotation and male pronucleus centration
Article Snippet: .. We performed co-injection of mRNA encoding mcherry-mTRIM21 (Addgene plasmid 105522) together with a monoclonal antibody targeting MRCKbeta (Abcam ab61328). ..

Article Title: A novel and critical role of the intracellular Zona Pellucida protein 2 (ZP2) for blastocyst formation in mice
Article Snippet: .. mRNA was synthesized from an expression construct (pGEMHE- mCherry - trim21 plasmid, Addgene Plasmid #105522). .. The plasmid was linearized with SwaI (cat. no. FD1244, Thermo Fisher Scientific, Karlsruhe, Germany).

Synthesized:

Article Title: A novel and critical role of the intracellular Zona Pellucida protein 2 (ZP2) for blastocyst formation in mice
Article Snippet: .. mRNA was synthesized from an expression construct (pGEMHE- mCherry - trim21 plasmid, Addgene Plasmid #105522). .. The plasmid was linearized with SwaI (cat. no. FD1244, Thermo Fisher Scientific, Karlsruhe, Germany).

Expressing:

Article Title: A novel and critical role of the intracellular Zona Pellucida protein 2 (ZP2) for blastocyst formation in mice
Article Snippet: .. mRNA was synthesized from an expression construct (pGEMHE- mCherry - trim21 plasmid, Addgene Plasmid #105522). .. The plasmid was linearized with SwaI (cat. no. FD1244, Thermo Fisher Scientific, Karlsruhe, Germany).

Construct:

Article Title: A novel and critical role of the intracellular Zona Pellucida protein 2 (ZP2) for blastocyst formation in mice
Article Snippet: .. mRNA was synthesized from an expression construct (pGEMHE- mCherry - trim21 plasmid, Addgene Plasmid #105522). .. The plasmid was linearized with SwaI (cat. no. FD1244, Thermo Fisher Scientific, Karlsruhe, Germany).



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